Premium
Interleukin‐11 as an osteoprotegerin‐inducing factor in culture medium of blastic cells from a patient with acute megakaryocytic leukemia complicated with osteosclerosis
Author(s) -
Sato Tsutomu,
Matsunaga Takuya,
Kida Masaya,
Morii Kazuhiro,
Machida Takuro,
Kawano Yutaka,
Nakamura Kiminori,
Kuribayashi Kageaki,
Takada Kohichi,
Iyama Satoshi,
Sato Yasushi,
Takayama Tetsuji,
Takahashi Minoru,
Kato Junji,
Chokki Manabu,
Niitsu Yoshiro
Publication year - 2004
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.20141
Subject(s) - osteoprotegerin , osteosclerosis , medicine , cytokine , leukemia , western blot , immunology , cancer research , interleukin , pathology , chemistry , receptor , biochemistry , activator (genetics) , gene
We came across a rare case of acute megakaryocytic leukemia, the clinical course of which was relatively chronic and nonaggressive. This case was complicated with generalized severe osteosclerosis (OS). The medium in which blastic cells from the patient were cultured showed a strong activity to enhance the expression of an osteosclerotic cytokine, osteoprotegerin (OPG), as revealed by real‐time quantitative RT‐PCR and Western blot analysis. The OPG‐inducing activity of the culture medium was neutralized by the anti‐interleukin‐11 (IL‐11) antibody. These results indicate that IL‐11 produced by the blasts was a causative factor of the OS observed in this patient. Am. J. Hematol. 77:62–66, 2004. © 2004 Wiley‐Liss, Inc.