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Molecular characterization of hemoglobin C in Thailand
Author(s) -
Sanchaisuriya Kanokwan,
Fucharoen Goonnapa,
Saeung Nattaya,
Siriratmanawong Nirut,
Surapot Sutja,
Fucharoen Supan
Publication year - 2001
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.1105
Subject(s) - hemoglobin , hemoglobin variants , polymerase chain reaction , globin , hemoglobinopathy , microbiology and biotechnology , hemoglobin e , haplotype , biology , hemoglobin a2 , compound heterozygosity , genetics , genomic dna , hemoglobin electrophoresis , gene , allele , biochemistry , hemolytic anemia , immunology
We describe hematologic and DNA characterization of 12 hemoglobin C heterozygotes and three compound heterozygotes for hemoglobin C and hemoglobin E found in Thailand. Amplification and DNA analysis of genomic DNA by the polymerase chain reaction procedure permitted the identification of the β C mutation at codon 6 of β‐globin gene (β 6; GAG–AAG). β‐Globin gene haplotype analysis demonstrated that all β C globin genes detected in these Thai individuals were associated with the haplotype (+ − − − − − +), indicating a non‐African origin of this abnormal hemoglobin in Thailand. On routine hemoglobin typing, hemoglobin C is usually mistakenly identified as hemoglobin E because of theirs similar mobilities on cellulose acetate electrophoresis. The simple DNA assay for hemoglobin C based on an allele‐specific polymerase chain reaction for accurate diagnosis of hemoglobin C was therefore developed. Am. J. Hematol. 67:189–193, 2001. © 2001 Wiley‐Liss, Inc.
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