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Correlation of hematopoietic progenitor cell count determined by the SE‐9000™ automated hematology analyzer with CD34 + cell count by flow cytometry in leukapheresis products
Author(s) -
Park Keon Uk,
Kim Sang Hee,
Suh Cheolwon,
Kim Shin,
Lee Sun Jong,
Park Jung Sun,
Cho Hwa Jung,
Kim Kang Wook,
Lee Keehyun,
Kim Hyo Jung,
Park Jinny,
Joo Min Young,
Kim Jeong Gyoon,
Kim Taewon,
Lee Je Hwan,
Kim Sung Bae,
Kim Sang We,
Lee Kyoo Hyung,
Lee Jung Shin,
Kim Woo Kun,
Park Chan Jeong,
Chi Hyun Sook
Publication year - 2001
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.1074
Subject(s) - leukapheresis , cd34 , flow cytometry , stem cell , haematopoiesis , blood cell , white blood cell , progenitor cell , medicine , hematology analyzer , peripheral blood mononuclear cell , hematology , immunology , cell , andrology , chemistry , biology , in vitro , biochemistry , genetics
The yield of stem cell collection after mobilization is crucial for autologous peripheral blood stem cell (PBSC) transplantation. Quantitative determinations of CD34 + cells using flow cytometry or stem cell culture have been used, but these methods require much time, technical experience, and expensive reagents. The automated hematology analyzer (Sysmex SE‐9000™, TOA, Japan) equipped with the Immature Information (IMI) channel for immature myeloid cells can detect IMI + cells within 90 sec. Detection is made possible by the combination of a special reagent system and direct current/radiofrequency biosensors. We studied the relation of IMI + cells and variable cell counts with CD34 + cell yield in autologous stem cell harvest. In a series of 32 patients (median age, 44 years; M:F = 11:21), 184 leukaphereses were performed after mobilization regimens with chemotherapy and G‐CSF or G‐CSF alone. Full blood cell counts were enumerated on peripheral blood (PB) samples taken prior to each leukapheresis. Mononuclear cell (MNC) and IMI + cell counts by automated hematology analyzer and flow cytometry based CD34 + cell yield were measured on the harvested product. The relationship among PB white blood cells (WBC), PB monocytes, IMI + cells, MNC, and CD34 + cell yield in a single leukapheresis was estimated by Pearson correlation analysis. PB WBC count showed no correlation with CD34 + cell yield in a single leukapheresis ( r = 0.02, P = 0.81). PB monocyte count showed a weak correlation ( r = 0.21, P = 0.01) and MNC in harvest also showed a weak correlation ( r = 0.36, P = 0.0001) with CD34 + cell yield. In contrast, CD34 + cell yield correlated well with IMI + cell count ( r = 0.68, P = 0.0001), and data could be fitted by a linear regression equation, y = 0.330 + 0.974 x. IMI + cell assay by the automated hematology analyzer correlated well with the CD34 + cell yield in a mobilized autologous stem cell harvest. The IMI + cell count might be used as a simple and efficient indicator of blood stem cell mobilization and collection. Am. J. Hematol. 67:42–47, 2001. © 2001 Wiley‐Liss, Inc.