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Cell fusion is not involved in the generation of giant cells in the Hodgkin‐Reed Sternberg cell line L1236
Author(s) -
Re Daniel,
Benenson Elena,
Beyer Marc,
Gresch Oliver,
Draube Andreas,
Diehl Volker,
Wolf Jürgen
Publication year - 2001
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.1068
Subject(s) - multinucleate , giant cell , cell fusion , cell culture , microbiology and biotechnology , biology , cell , genetics
The mechanism of multinucleated cell formation in Hodgkin's disease has not yet been elucidated. We asked whether the giant multinucleated cells of the H‐RS cell line L1236 develop via fusion of the predominant smaller cells. As a positive control for the fusion assay, human B cells from the B‐cell lymphoma cell line BJA‐B were split into two fractions, stained with the fluorochromes CMTMR and CMFDA, respectively, and fused using the polyethylene glycol 1500 cell hybridization protocol. Double‐stained cells indicating fusion of BJA‐B cells were detectable for up to 5 days. In parallel, L1236 cells were split into two fractions, stained with the fluorochromes, and mixed. No double‐stained L1236 cells were detected. The same result was obtained when using FACS‐sorted small mononuclear L1236 cells. It is thus concluded that the large multinucleated cells of the monoclonal H‐RS cell line L1236 have emerged by endomitosis rather than by spontaneous cell fusion. Am. J. Hematol. 67:6–9, 2001. © 2001 Wiley‐Liss, Inc.