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Simple fluorescent PCR method for detection of large deletions in the β‐globin gene cluster
Author(s) -
De Andrade Tiago Gomes,
Saad Sara Teresinha Olalla,
de Fatima Sonati Maria,
Costa Fernando Ferreira
Publication year - 2003
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.10291
Subject(s) - biology , gene cluster , gene , computational biology , genetics , microbiology and biotechnology , cluster (spacecraft) , polymerase chain reaction , computer science , programming language
We developed a semi‐automated approach to detect large deletions in the β‐globin gene cluster, based on the quantitative differences in the amplifications of samples by a fluorescent PCR. With this strategy, we were able to detect the presence of HPFH‐2 in an African‐Brazilian subject, confirmed by sequencing analysis. Differently from other PCR strategies, GAP‐PCR for example, it has the potential to identify new deletions. Am. J. Hematol. 72:225–227, 2003. © 2003 Wiley‐Liss, Inc.