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Rapid diagnosis of invasive pulmonary aspergillosis by quantitative polymerase chain reaction using bronchial lavage fluid
Author(s) -
Kawazu Masahito,
Kanda Yoshinobu,
Goyama Susumu,
Takeshita Masataka,
Nannya Yasuhito,
Niino Miyuki,
Komeno Yukiko,
Nakamoto Tetsuya,
Kurokawa Mineo,
Tsujino Shiho,
Ogawa Seishi,
Aoki Katsunori,
Chiba Shigeru,
Motokura Toru,
Ohishi Nobuya,
Hirai Hisamaru
Publication year - 2003
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.10259
Subject(s) - bronchoalveolar lavage , polymerase chain reaction , aspergillosis , aspergillus , galactomannan , real time polymerase chain reaction , aspergillus fumigatus , dna , pathology , medicine , biology , microbiology and biotechnology , immunology , lung , gene , biochemistry , genetics
Polymerase chain reaction (PCR) is a sensitive method for detection of Aspergillus DNA in bronchoalveolar lavage fluid, but it has not yet been able to distinguish infection from contamination. We have established a technique to quantify Aspergillus DNA using a real‐time PCR method to resolve this problem, and we report herein a successful application of real‐time PCR to diagnose invasive pulmonary aspergillosis by comparing the amount of Aspergillus DNA in bronchial lavage fluid from an affected area to that from an unaffected area. This novel tool will provide rapid, sensitive, and specific diagnosis of pulmonary aspergillosis. Am. J. Hematol. 72:27–30, 2003. © 2002 Wiley‐Liss, Inc.