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Lack of allelic exclusion and isotype switching in B cell chronic lymphocytic leukemia
Author(s) -
Katayama Yuta,
Sakai Akira,
Katsutani Shinya,
Takimoto Yasuo,
Kimura Akiro
Publication year - 2001
Publication title -
american journal of hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.456
H-Index - 105
eISSN - 1096-8652
pISSN - 0361-8609
DOI - 10.1002/ajh.10042
Subject(s) - allelic exclusion , chronic lymphocytic leukemia , isotype , biology , allele , microbiology and biotechnology , gene , immunoglobulin class switching , immunoglobulin d , polymerase chain reaction , genetics , immunoglobulin heavy chain , b cell , leukemia , antibody , t cell receptor , t cell , immune system , monoclonal antibody
Two rearranged bands of the IgH gene were detected in a case with B cell chronic lymphocytic leukemia (B‐CLL). The expressed VH gene was only VH1 with no somatic mutations in IgM and IgD. The expressions of Cμ, Cδ, and Cγ were detected by reverse transcription of RNA followed by the polymerase chain reaction (RT‐PCR). Sequence analysis of the CDR3 regions of each PCR reaction product showed that the sequence of one rearranged allele was identical to those of the expressed VH1 gene, Cμ and Cδ, and the sequence of another rearranged allele was identical to that of Cγ. However, none of the expressed VH genes was detected in IgG. These findings suggest that this is a case of B‐CLL lacking allelic exclusion and undergoing a class switch of one allele with the incomplete expression of the VH gene. Am. J. Hematol. 68:295–297, 2001. © 2001 Wiley‐Liss, Inc.