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Tissue‐nonspecific alkaline phosphatase participates in the establishment and growth of feather germs in embryonic chick skin cultures
Author(s) -
Crawford Karen,
Weissig Helge,
Binette François,
Millán José Luis,
Goetinck Paul F.
Publication year - 1995
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/aja.1002040107
Subject(s) - alkaline phosphatase , biology , feather , mesoderm , phosphatase , microbiology and biotechnology , embryonic stem cell , gene , genetics , enzyme , biochemistry , zoology
Alkaline phosphatase activity is present in the mesoderm of embryonic chick skin and becomes spatially restricted to the dermal condensation of the developing feather germs. Inhibitors to tissue‐nonspecific (liver/bone/kidney), but not intestinal, alkaline phosphatase inhibit the establishment and growth of feather germs in cultured skins. A window of maximum sensitivity to the inhibitor was observed to be the first day of culture when early development and establishment of pattern takes place. The cDNA for the avian tissue‐nonspecific alkaline phosphatase was cloned and sequenced, and Southern analysis revealed a single copy of this gene in the avian genome. Northern analysis revealed that a 2.8 kb transcript for this form of alkaline phosphatase is present in developing skin. © 1995 wiley‐Liss, Inc.