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Gsh ‐ 1 : A novel murine homeobox gene expressed in the central nervous system
Author(s) -
Valerius M. Todd,
Li Hung,
Stock Jeffrey L.,
Weinstein Michael,
Kaur Satbir,
Singh Gurparkash,
Potter S. Steven
Publication year - 1995
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/aja.1002030306
Subject(s) - biology , homeobox , homeobox a1 , central nervous system , gene , microbiology and biotechnology , genetics , anatomy , neuroscience , gene expression
We report the characterization of Gsh ‐ 1 , a novel murine homeobox gene. Northern blot analysis revealed a transcript of approximately 2 kb in size present at embryonic days 10.5, 11.5, and 12.5 of development. The cDNA sequence encoded a proline rich motif, a polyalanine tract, and a homeodomain with strong homology to those encoded by the clustered Hox genes. The Gsh ‐ 1 expression pattern was determined for days E8.5 to E13.5 by whole mount and serial section in situ hybridizations. Gsh ‐ 1 transcription was restricted to the central nervous system. Expression is present in the neural tube and hindbrain as two continuous, bilaterally symmetrical stripes within neural epithelial tissue. In the mesencephalon, expression is seen as a band across the most anterior portion. There is also diencephalon expression in the anlagen of the thalamus and the hypothalamus as well as in the optic stalk, optic recess, and the ganglionic eminence. Moreover, through the use of fusion proteins containing the Gsh ‐ 1 homeodomain, we have determined the consensus DNA ninding site of the Gsh ‐ 1 homeoprotein to be GC T / C A / C ATTA G / A . ©1995 Wiley‐Liss, Inc.