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Developmental expression of fibroblast growth factor receptor‐1 ( cek‐1; flg ) during heart development
Author(s) -
Sugi Yukiko,
Sasse Joachim,
Barron Matthew,
Lough John
Publication year - 1995
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/aja.1002020203
Subject(s) - biology , microbiology and biotechnology , fibroblast growth factor , expression (computer science) , receptor , fibroblast , fibroblast growth factor receptor 3 , fibroblast growth factor receptor 2 , fibroblast growth factor receptor , medicine , endocrinology , genetics , cell culture , computer science , programming language
Abstract Previous work in this laboratory has indicated that fibroblast growth factor‐2 (FGF‐2; bFGF) regulates the initial stages of avian heart development in paracrine and autocrine fashion (Parlow et al. [1991] Dev. Biol. 146:139–147; Sugi et al. [1993] Dev. Biol. 157:28–37). Because these findings inferred the presence of a functional receptor for fibroblast growth factor (FGFR), we have immunochemically assessed the appearance of FGFR‐1 ( cek ‐ 1 ; flg ) during development. Using a peptide‐generated antibody, Western blots of total embryonic proteins revealed that FGFR‐1 was barely detectable at pre‐heart stages, followed by sequential increases in relative abundance that peaked at stage 24, followed by a decline at days 7–14. Western blots of proteins from isolated embryonic hearts demonstrated a similar developmental pattern, except that FGFR‐1 expression was not decreased at later stages. The presence of FGFR‐1 mRNA was verified by reverse transcription/polymerase chain reaction (RT/PCR) amplification. Immuno‐histochemical examination revealed punctate deposits of FGFR‐1 in the precardiac endoderm at stage 6, followed by detection in the endoderm, foregut, and pre‐cardiac splanchnic mesoderm at stage 8 and in the newly formed myocardium at the heart tube stage (9/10). By stage 13, FGFR‐1 staining was observed only in the myocardium, a pattern which persisted at least until stage 30 (day 7), after which only isolated hearts were examined. After stage 30, staining was diminished in the ventricle, but not in the atrium. Staining of cardiac endothelial cells was not observed at any stage. A functional role for FGFR‐1 was indicated by experiments in which anti‐FGFR‐1, but not pre‐absorbed antiserum, retarded proliferation and multilayering of cardiogenic cells in an in vitro model of cardiac morphogenesis. © Wiley‐Liss, Inc.