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Complex patterns of expression suggest extensive roles for the α 2 β 1 integrin in murine development
Author(s) -
Wu Justina E.,
Santoro Samuel A.
Publication year - 1994
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/aja.1001990405
Subject(s) - biology , integrin , microbiology and biotechnology , extracellular matrix , complementary dna , in situ hybridization , collagen receptor , fibronectin , laminin , integrin, beta 6 , immunocytochemistry , g alpha subunit , alpha (finance) , protein subunit , gene expression , receptor , endocrinology , gene , biochemistry , medicine , construct validity , nursing , patient satisfaction
The extracellular matrix plays important roles in embryogenesis. The integrin family of adhesion receptors may mediate critical cellular interactions with the extracellular matrix during development. In this study, we elucidated the developmental spatial and temporal expression pattern of the α 2 β 1 integrin heterodimer, a cell surface receptor for collagens and laminin. We generated reagents for studying the α 2 β 1 integrin and examined the developmental expression of the integrin in postimplantation mice. A partial length murine α 2 cDNA was isolated and the protein encoding region was found to be 82% homologous to that of the human α 2 cDNA. A synthetic peptide corresponding to the carboxy‐terminus of murine α 2 was used to generate α 2 ‐specific antiserum. The antiserum and riboprobes derived from both the α 2 cDNA and the previously characterized murine β 1 subunit cDNA were used to determine the spatiotemporal expression of the α 2 subunit by immunocytochemistry and of the α 2 and β 1 mRNAs by in situ hybridization. Both approaches gave concordant results. Expression of the α 2 integrin subunit was observed in both the maternal and embryonic components of the placenta, namely the perivascular and basal zone decidual cells and decidual cells and spongiotrophoblasts at the maternal/embryonic junction. Expression was also observed in cells actively producing and remodeling the extracellular matrix in the maternal uterus and in the developing gut, lens, cartilage, bone, and tooth of the embryo. Generally, expression of the α 2 integrin subunit was found in cells entering their later stages of differentiation such as in chondrocytes as they became hypertrophic, ameloblasts and odontoblasts as they became columnar and began to secrete the matrix of the tooth, endothelial cells after they formed tubules, in the lens just prior to and during lens fiber production, and in the collecting ducts of the kidney only after full gestation. Throughout embryogenesis, β 1 mRNA was widely distributed and present in cell types expressing α 2 mRNA and protein. The developmental expression pattern of the α 2 β 1 integrin suggests roles for the integrin in placental development and matrix assembly and remodeling. © 1994 Wiley‐Liss, Inc.