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Developmental regulation and cell type‐specific expression of the murine γ F‐crystallin gene is mediated through a lens‐specific element containing the γ F‐1 binding site
Author(s) -
Goring Daphne R.,
Bryce Dawn Marie,
Tsui LapChee,
Breitman Martin L.,
Liu Qiurong
Publication year - 1993
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/aja.1001960208
Subject(s) - biology , enhancer , crystallin , lens fiber , gene expression , gene , microbiology and biotechnology , lens (geology) , regulation of gene expression , regulatory sequence , genetics , paleontology
The mouse γF‐crystallin gene, one of six differentially regulated members of the γ‐crystallin gene family, is expressed exclusively in central nuclear fiber cells of the adult lens. The expression of this gene is controlled through regulatory elements contained in two upstream enhancers and the proximal promoter. Here we show that while the upstream enhancers and the proximal promoter could each direct gene expression in fiber cells formed at early stages of lens growth and development, cooperation between these elements is required to achieve expression in fiber cells formed at later stages. Evidence is provided that cooperative interaction between these elements modulates gene expression by increasing promoter strength. We also show that sequences within the proximal promoter region that bind lens cell nuclear factor γF‐1 are sufficient to elicit gene expression in central nuclear fiber cells of the adult lens. © 1993 wiley‐Liss, Inc.