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Mullerian inhibiting substance binding and uptake
Author(s) -
Catlin E. A.,
Ezzell R. M.,
Donahoe P. K.,
Manganaro T. F.,
Ebb R. G.,
MacLaughlin D. T.
Publication year - 1992
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/aja.1001930402
Subject(s) - biology , endocytosis , confocal microscopy , microbiology and biotechnology , receptor , fluorescein , ligand (biochemistry) , confocal , cytosol , fluorescence , biochemistry , physics , geometry , mathematics , quantum mechanics , enzyme
Mullerian inhibiting substance (MIS) is a 140,000 M r Sertoli cell derived glycoprotein with a critical regulatory role in the male fetus initiated presumably by ligand binding with receptor. To localize this binding species we performed time course incubations of cultured fetal rat lungs or control tissues with MIS, applied rabbit anti‐MIS IgG, and fluorescein conjugated antirabbit IgG, and examined specimens with laser confocal microscopy. Punctate surface fluorescence followed by cytosolic and nuclear localization in lung consistent with specific adsorptive endocytosis was seen. Confocal imaging also detected MIS binding to the Mullerian duct in the urogenital ridge. Crosslinking of 125 I‐MIS with plasma membranes revealed a high molecular mass binder with signal displaceable by excess unlabeled ligand. These data support the hypothesis that a specific plasma membrane binding protein for MIS exists. © 1992 Wiley‐Liss, Inc.