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Protein ion‐exchange adsorption kinetics
Author(s) -
Wesselingh J. A.,
Bosma J. C.
Publication year - 2001
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690470710
Subject(s) - chemistry , adsorption , ionic strength , diffusion , kinetics , ion exchange , steric effects , surface diffusion , thermodynamics , inverse , chromatography , protein adsorption , ion , analytical chemistry (journal) , chemical physics , stereochemistry , organic chemistry , aqueous solution , physics , geometry , mathematics , quantum mechanics
The kinetics of the adsorption of the protein BSA on the ion exchanger Q‐Sepharose FF were measured for several values of the pH and ionic strength, using several techniques. The measurements were best described with a model incorporating both surface and pore diffusion and with the chemical potential gradient as the driving force for diffusion. The surface‐diffusion coefficients from this model show an inverse exponential dependency on the binding strength. This dependency can be explained by an activated jump mechanism. The pore‐diffusion coefficient is much lower than that in free solution, which is probably caused by a combination of steric and electric exclusion.