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pH dependence of ion‐exchange equilibrium of proteins
Author(s) -
Bosma J. C.,
Wesselingh J. A.
Publication year - 1998
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690441108
Subject(s) - chemistry , ionic strength , equilibrium constant , ion exchange , adsorption , dynamic equilibrium , ionic bonding , ion , thermodynamics , bovine serum albumin , chemical equilibrium , chromatography , inorganic chemistry , organic chemistry , physics , aqueous solution
The adsorption equilibrium of bovine serum albumin on Q‐sepharose, a strong union exchanger, was studied with batch equilibrium experiments at pH between 4 and 9 and ionic strengths between 5 and 440 mmol/L. Dependence of the adsorption equilibrium on the ionic strength was modeled as an ion exchange reaction. A simplified mechanism of this ion exchange reaction also yielded an expression for the dependence of the equilibrium on the charge of the protein. This model describes the measurements well, using fitted constants with physically realistic values.