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Rate parameters and gradient correlations for gradient‐elution chromatography
Author(s) -
Luo Robert G.,
Hsu James T.
Publication year - 1997
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690430219
Subject(s) - gradient elution , elution , chromatography , ion chromatography , chemistry , concentration gradient , work (physics) , gradient method , experimental data , analytical chemistry (journal) , high performance liquid chromatography , mathematics , algorithm , statistics , physics , thermodynamics
Rate parameters and gradient correlations of proteins are very important to engineering investigations of gradient‐elution chromatography. Before carrying out computer simulations for gradient process, these rate parameters and gradient correlations must be determined from experimental data. This work presents a systematic method for estimating and determining these parameters and correlations using experimental data and computer‐simulation results based on isocratic runs. The values determined were then employed in gradient‐elution studies by putting them into the rate model to perform computer simulations. Experimental runs under gradient conditions using the parameters and correlations just referred to were carried out in a DEAE Sepharose CL‐6B ion‐exchange chromatography. Comparisons between the experimental data and the simulation results show good agreement.