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Modeling mass transfer with enzymatic reaction in electrochemical multilayer microreactors
Author(s) -
Bacha Saliha,
Montagné Marielle,
Bergel Alain
Publication year - 1996
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690421024
Subject(s) - microreactor , mass transfer , electrochemistry , chemistry , chemical engineering , materials science , chromatography , organic chemistry , catalysis , electrode , engineering
Electrochemical biosensors were used to develop a numerical model of transient mass transfer coupled with enzymatic reactions in thin multilayer microstructures. A finite volume method integrates the partial derivative mass‐balance equations making the model very versatile. With a multilayer physical description, general nonlinear enzymatic kinetics and efficient space discretization, the model could be applied to various devices under a broad range of physicochemical conditions. Theoretical results were validated according to experimental data obtained with three different biosensors involving homogeneous or heterogeneous enzymatic catalyses. Model predictions are further discussed for a bienzymatic immobilized enzyme system. The biosensor transient behavior depended strongly on the location of the enzymes in the device and on the mass‐transport features. A transient maximum on the current‐time curves was predicted accurately by the model. This could be used to improve the biosensor performance. The model can also be a useful framework for designing new electrochemical microreactors.