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Modeling gradient elution of proteins in ion‐exchange chromatography
Author(s) -
Gallant Stuart R.,
Vunnum Suresh,
Cramer Steven M.
Publication year - 1996
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690420911
Subject(s) - elution , chemistry , chromatography , gradient elution , ion exchange , ion chromatography , steric effects , work (physics) , adsorption , analytical chemistry (journal) , ion , high performance liquid chromatography , thermodynamics , physics , organic chemistry
The steric mass action (SMA) model of ion exchange is employed in concert with appropriate mass balance equations to describe the behavior of a concentrated band of protein under gradient‐elution conditions. A method of characteristics approach is employed to solve the model equations rapidly. The model is shown to accurately predict the gradient elution of the protein cytochrome C in a strong cation‐exchange column. This work constitutes an advance over previously available models as it allows engineers to rapidly consider the effects of nonlinear adsorption in gradient‐elution chromatography using a spreadsheet. Further, it provides significant physical insight into the process of gradient elution for preparative and large‐scale separations.