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Protein separation by continuous‐flow electrophoresis in microgravity
Author(s) -
Clifton Michael J.,
Balmann Hélène RouxDe,
Sanchez Victor
Publication year - 1996
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690420727
Subject(s) - electrophoresis , chromatography , separation (statistics) , separation process , resolution (logic) , flow (mathematics) , separation method , electrohydrodynamics , high resolution , chemistry , free flow electrophoresis , continuous flow , protein purification , analytical chemistry (journal) , mechanics , physics , computer science , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , geology , biochemistry , electrode , enzyme , remote sensing , artificial intelligence , machine learning
During the IML‐2 space shuttle mission, the RAMSES instrument was operated in the Spacelab module. This continuous‐flow electrophoresis device performs separation and purification of protein solutions on a preparative scale. Samples containing artificial mixtures of pure proteins were used to test the capabilities of the device, and useful separations were obtained for proteins having a mobility difference of only 3 × 10 −9 m 2 ·V −1 ·s −1 . Operating conditions that cannot be applied on earth were explored for two different sample concentrations, one of which is too high to allow treatment on earth. It agrees well with a previously published numerical model in that the main cause of loss in resolution in this process is the electrohydrodynamic spreading of the protein filaments.