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Extension of metabolic control analysis to chemostat bioreactors
Author(s) -
Tan Hiong Leong,
Delgado Javier,
Liao James C.
Publication year - 1996
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690420526
Subject(s) - chemostat , bioreactor , steady state (chemistry) , substrate (aquarium) , control theory (sociology) , biological system , product inhibition , batch reactor , chemistry , process engineering , computer science , engineering , control (management) , biology , ecology , biochemistry , genetics , non competitive inhibition , organic chemistry , artificial intelligence , bacteria , enzyme , catalysis
Abstract A method of determining control coefficients and pseudo‐first‐order kinetic parameters is presented for cell growth, substrate consumption, and product formation in a chemostat bioreactor. From the equations that relate the control coefficients to process variables, such as feed concentrations of the limiting substrate and the product of interest, a perturbation method is developed to determine control coefficients from steady‐state measurements. This method combined with the transient response analysis provides a practical way for determining control coefficients and estimating kinetic properties in chemostat bioreactor systems. To determine the kinetic parameters, one measures cell mass, concentrations of the limiting substrate and the product of interest following a step change in the feed concentration until the system reaches a new steady state. The time courses of these variables are processed to obtain control coefficients, which yield the pseudo‐first‐order kinetic constants for cell growth, substrate consumption, and product formation. Only steady‐state responses are needed in calculation, if a step change in cell concentration in the feed stream can also be introduced without significantly perturbing cell physiology. This method is useful in characterizing the kinetics of whole cell bioreactions: results from chemostat experiments can be used to design operating strategies for batch or fed‐batch bioreactions. It is generally applicable to continuous‐stir‐tank reactors with interacting parallel reactions.