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Protein transport in ultrafiltration hollow‐fiber bioreactors
Author(s) -
Patkar Anant Y.,
Koska Jürgen,
Taylor David G.,
Bowen Bruce D.,
Piret James M.
Publication year - 1995
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690410222
Subject(s) - concentration polarization , transferrin , chemistry , pressure drop , bovine serum albumin , osmotic pressure , biophysics , bioreactor , blood proteins , chromatography , membrane , materials science , mechanics , biochemistry , biology , physics , organic chemistry
A 1‐D model, which neglects radial variations, describes the hydrodynamics of cell‐free ultrafiltration hollow‐fiber bioreactors (HFBRs) and the transport of highmolecular‐weight proteins trapped in the extracapillary space (ECS). The profiles of radially‐averaged protein concentrations predicted by this model are identical to those obtained using a model with radial variations. The model predictions agree well with axial profiles of bovine serum albumin (BSA) and human transferrin concentrations measured in transient and steady‐state experiments. The validated model explores the influence of cell culture operating conditions on HFBR protein transport. Increasing protein loading decreases BSA and transferrin polarization in HFBRs operated with unidirectional lumen flow. A relationship developed predicts the protein loading needed to ensure a nonzero steady‐state protein concentration throughout the ECS. This critical protein loading depends only on the lumen pressure drop and the ECS protein osmotic pressure. Periodic reversal of the lumen flow direction also decreases protein polarization. The influence of the flow‐direction switching time and membrane permeability on the ECS protein distribution is investigated.