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A kinetic model for the fungal pellet lifecycle
Author(s) -
Michel Frederick C.,
Grulke Eric A.,
Reddy C. Adinarayana
Publication year - 1992
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690380915
Subject(s) - phanerochaete , mycelium , pellets , respirometer , secondary metabolism , chemistry , manganese peroxidase , metabolism , pellet , respiration , biomass (ecology) , lignin , oxygen , bioreactor , botany , food science , environmental chemistry , biochemistry , biology , peroxidase , ecology , paleontology , enzyme , organic chemistry , biosynthesis
Many industrially significant microbial products are produced during secondary metabolism by fungal pellets. Most models of mycelial pellet growth, however, consider only the primary growth phase. During secondary metabolism, the whiterot fungus Phanerochaete chrysosporium produces extra‐cellular lignin peroxidases and manganese‐dependent peroxidases which have been implicated in the degradation of lignin and various xenobiotics. This unstructured kinetic model for mycelial pellet cultures of P. chrysosporium describes the culture dry weight, respiration, glucose consumption, nutrient nitrogen uptake, pellet size and oxygen effectiveness factor during the lag, primary growth, secondary metabolic, and death phases. During secondary metabolism, the intraparticle diffusion of oxygen limits metabolism. Parameters for respiration during secondary metabolism are validated using three separate measurements: 1. by measuring oxygen concentration profiles in mycelial pellets using oxygen microelectrodes; 2. by measuring the depletion of oxygen from the culture fluid using a respirometer; and 3. by measuring the daily evolution of carbon dioxide. The physiological state of the mycelial pellets is predicted for various culture conditions and is related to the production of LIP and MNP during secondary metabolism.