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Immobilization of enzymes in porous solids under restricted diffusion conditions
Author(s) -
Hossain Md. M.,
Do D. D.,
Bailey J. E.
Publication year - 1986
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690320705
Subject(s) - glucose oxidase , diffusion , immobilized enzyme , porosity , chemistry , porous glass , diffusion process , constant (computer programming) , thermodynamics , porous medium , kinetics , process (computing) , chemical engineering , materials science , chromatography , enzyme , organic chemistry , computer science , engineering , physics , innovation diffusion , knowledge management , programming language , operating system , quantum mechanics
A restricted diffusion model is developed to study the immobilization of enzyme in porous solid supports. Simulation studies have been carried out for various combinations of process variables and parameters of the immobilization system. The model has also been used to develop a method for estimating the intrinsic rate constant of immobilization when enzyme diffusion into the support is restricted. Results of experiments in which glucose oxidase was immobilized in porous glass supports are consistent with model simulations.