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Prediction of adsorption and desorption of protein on dextran based ion‐exchange resin
Author(s) -
Tsou HaiShung,
Graham E. E.
Publication year - 1985
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690311205
Subject(s) - desorption , adsorption , chemistry , chromatography , kinetics , bovine serum albumin , dextran , sephadex , ion exchange resin , langmuir adsorption model , ion exchange , chemical engineering , ion , inorganic chemistry , organic chemistry , physics , quantum mechanics , engineering , enzyme
The equilibrium and kinetic adsorption and desorption of bovine serum albumin (BSA), fraction V, on Sephadex A‐50 has been studied. The equilibrium data have been presented by a Langmuir isotherm for both adsorption and desorption conditions. The kinetics of adsorption in a batch system may be represented by a simple diffusional model. The kinetics of desorption of BSA in a batch system show a very fast initial rate due to the rapid shrinkage of the resin with changes in salt concentration. This has been modeled by including a protein exclusion term in the kinetics for desorption. The above information has been used to predict the breakthrough of BSA in a column operation. Under the pressure exerted in a column flow system the Sephadex A‐50 is severely compressed. The effect this has on the breakthrough rate of BSA has been demonstrated.