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Electropolarization chromatography
Author(s) -
Reis Joaquim F. G.,
Lightfoot E. N.
Publication year - 1976
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690220423
Subject(s) - fractionation , ultrafiltration (renal) , chromatography , electrolyte , chemistry , process (computing) , biological system , computer science , biology , electrode , operating system
The utility of electropolarization chromatography for fractionation of native protein mixtures is examined both mathematically and experimentally. Mathematical prediction based on a simplified but basically realistic model of this process suggests it to be very promising for both analytical and preparative purposes. This expectation is borne out by tests with well‐defined proteins in single ultrafiltration fibers. The major observed departure from model prediction is an unexpectedly sharp increase in retardation at a critical voltage specific to the protein and composition of the carrier electrolyte. This permits sharper separations than previously expected and is thus favorable.