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Theoretical plate concept in chromatography: Part II
Author(s) -
Said A. S.
Publication year - 1959
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.690050115
Subject(s) - elution , chromatography , column (typography) , chemistry , continuation , simple (philosophy) , volume (thermodynamics) , mathematics , expression (computer science) , geometry , thermodynamics , physics , computer science , philosophy , epistemology , connection (principal bundle) , programming language
Part A of this paper is essentially a continuation of a previous paper on the theoretical plate concept in chromatography ( 4 ). It deals with some special cases of eluting conditions and zone shapes. A more general equation, which combines both elution and deposition, is derived, and methods for approximating a continuous zone by means of a discontinuous one are discussed. Part B discusses the effect of the finite size of samples and the dead free volume at the bottom of the column on the shape of elution curves. The effect of representative sample volumes is calculated, and the deviation between the experimental and the true elution curves is illustrated. A simple expression for the number of theoretical plates utilizing the ratio between the maximum concentrations at the top and bottom of the column is derived and corrected for the effect of the finite size of samples.