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Screen‐printing of nanocube‐based flexible microchips for the precise biosensing of ethanol during fermentation
Author(s) -
Zhang Sijian,
Xie Ying,
Feng Jingyu,
Chu Zhenyu,
Jin Wanqin
Publication year - 2021
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.17142
Subject(s) - fermentation , biosensor , chemistry , nicotinamide adenine dinucleotide , ethanol , alcohol dehydrogenase , nanotechnology , screen printing , electrocatalyst , ethanol fermentation , nanocomposite , linear range , chromatography , materials science , electrode , detection limit , organic chemistry , nad+ kinase , electrochemistry , enzyme , composite material
The real‐time and full concentration analysis of ethanol during the fermentation reaction allows the precise control of the microbial metabolism to promote the conversion rate; however, only few techniques can directly detect the fermentation broth without pretreatment. To address this issue, a screen‐printed biosensing microchip (SPBM) was proposed to analyze the ethanol concentration in fermentation, relying on designing a well‐defined nanocubic structure of an Au nanoparticles/nickel hexacyanoferrate nanocomposite. This nanocomposite was then made into a printing ink for the direct fabrication of a SPBM. The as‐prepared microchip exhibits an ultrahigh electrocatalysis on nicotinamide adenine dinucleotide with a high sensitivity of 96.2 ± 2.9 μA·mM −1 ·cm −2 . Moreover, after the immobilization of alcohol dehydrogenase, the accurate and rapid monitoring of the ethanol concentration during the real fermentation reaction can be realized within an ultrawide linear range of 0.1–10 M in the broth without any pretreatment.