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Proteome editing using engineered proteins that hijack cellular quality control machinery
Author(s) -
LopezBarbosa Natalia,
Ludwicki Morgan B.,
DeLisa Matthew P.
Publication year - 2020
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.16854
Subject(s) - proteome , computational biology , biology , genome editing , crispr , function (biology) , gene silencing , transcription activator like effector nuclease , rna interference , microbiology and biotechnology , rna , bioinformatics , genetics , gene
Abstract Protein silencing is an important aspect of both scientific investigation of native protein function and therapeutic targeting of aberrant protein activity. Many techniques for silencing proteins at the DNA or RNA level exist such as CRISPR, RNAi, or TALEN. Cellular proteins can also be selectively removed at the posttranslational level using proteome editing techniques, many of which employ engineered proteins to engage natural cellular quality control machinery for accelerating the removal of otherwise stable proteins. Here, we summarize recent progress in the development of such engineered proteins, comparing them to analogous microbial‐, viral‐, and small molecule‐based strategies for selectively degrading proteins of interest. Finally, we highlight the advantages and limitations of proteome editing technologies and discuss how the application of directed evolution could alleviate current challenges and usher in a new wave of designer proteins for diagnostic and therapeutic application.