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An Arduino based automatic pressure evaluation system to quantify growth of non‐model anaerobes in culture
Author(s) -
Wilken St. Elmo,
Leggieri Patrick A.,
KerdmanAndrade Corey,
Reilly Matthew,
Theodorou Michael K.,
O'Malley Michelle A.
Publication year - 2020
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.16540
Subject(s) - arduino , anaerobic exercise , cabin pressurization , process engineering , fermentation , computer science , biomass (ecology) , bacterial growth , biochemical engineering , environmental science , biological system , simulation , biology , mechanical engineering , food science , engineering , ecology , embedded system , bacteria , physiology , genetics
Measuring the growth rate of non‐model anaerobic microbes typically requires the use of time‐consuming and often destructive manual measurements. Here, an Arduino based automatic pressure evaluation system (A‐APES) was developed to automatically measure the rate of fermentation gas production as a proxy for microbial growth in anaerobic systems. The A‐APES system measures accumulated gas pressure in sealed cultures accurately at high‐resolution, while venting the system at programmed intervals to prevent over pressurization. The utility of A‐APES is demonstrated in this study by quantifying the growth rate and phases of a biomass‐degrading anaerobic gut fungus, which cannot be otherwise measured via conventional techniques due to its association with particulate substrates. Given the utility of the A‐APES approach, we provide a complete construction guide to fabricate the device, which is three times less expensive compared to existing commercial alternatives.