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Bioinformatic analysis of Chinese hamster ovary host cell protein lipases
Author(s) -
MacDonald Madolyn L.,
Hamaker Nathaniel K.,
Lee Kelvin H.
Publication year - 2018
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.16378
Subject(s) - chinese hamster ovary cell , computational biology , gene knockout , gene , biology , genome , genetics , cell culture
Complete, accurate genome assemblies are necessary to design targets for genetic engineering strategies. Successful gene knockdowns and knockouts in Chinese hamster ovary (CHO) cells may prevent the expression of difficult‐to‐remove host cell proteins (HCPs). HCPs, if not removed, can cause problems in stability, safety, and efficacy of the biotherapeutic. A significantly improved Chinese hamster (CH) reference genome was used to identify new knockout targets with similar predicted functions and characteristics as the difficult‐to‐remove host cell lipases, LPL, PLBL2, and LPLA2. The CHO‐K1 gene and protein sequences of several of these lipases were corrected using the updated CH genome. Sequence alignments were then used to identify conserved regions that may serve as possible targets for multiple simultaneous gene knockouts. Finally, the comparison of the CHO‐K1 lipase protein sequences to their human orthologs provided insight into which lipases, if persistent in the drug product, could possibly cause immunogenic responses in patients. Topical heading: Biomolecular Engineering, Bioengineering, Biochemicals, Biofuels, and Food. © 2018 American Institute of Chemical Engineers AIChE J , 64: 4247–4254, 2018