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Production of ethylene glycol from xylose by metabolically engineered Escherichia coli
Author(s) -
Chae Tong Un,
Choi So Young,
Ryu Jae Yong,
Lee Sang Yup
Publication year - 2018
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.16339
Subject(s) - xylose , escherichia coli , metabolic engineering , ethylene glycol , glycolaldehyde , chemistry , biochemistry , xylose metabolism , strain (injury) , bioprocess , caulobacter crescentus , enzyme , fermentation , biology , gene , organic chemistry , bacterial protein , catalysis , paleontology , anatomy
Ethylene glycol (EG) is an important chemical used for several industrial applications including poly(ethylene terephthalate) synthesis. In this study, Escherichia coli was metabolically engineered to efficiently produce EG from xylose. To biosynthesize EG, the Dahms pathway was introduced by expressing xylBC genes from Caulobacter crescentus ( xylBC ccs ). Various E. coli strains and glycolaldehyde reductases were screened to find E. coli W3110 strain and glycolaldehyde reductase ( yqhD ) as optimal combination for EG production. In silico genome‐scale metabolic simulation suggested that increasing the native xylose pathway flux, in the presence of the overexpressed Dahms pathway, is beneficial for EG production. This was achieved by reducing the Dahms pathway flux by employing a synthetic small regulatory RNA targeting xylB ccs . Fed‐batch culture of the final engineered E. coli strain produced 108.2 g/L of EG in a xylose minimal medium. The yield on xylose and EG productivity were 0.36 g/g (0.87 mol/mol) and 2.25 g/L/h, respectively. © 2018 American Institute of Chemical Engineers AIChE J , 64: 4193–4200, 2018