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Acid hydrolysis of glycosidic bonds in oat β‐glucan and development of a structured kinetic model
Author(s) -
Nguyen Hoang S. H.,
Hein Jari,
Sainio Tuomo
Publication year - 2018
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.16147
Subject(s) - glycosidic bond , cellobiose , hydrolysis , chemistry , glucan , homogeneous , reactivity (psychology) , kinetic energy , acid hydrolysis , stereochemistry , polysaccharide , computational chemistry , organic chemistry , thermodynamics , enzyme , cellulase , medicine , physics , alternative medicine , pathology , quantum mechanics
Homogeneous acid‐catalyzed hydrolysis of oat β‐glucan, which contains β‐(1,4) and β‐(1,3) glycosidic bonds in a nonrandom order, was studied at 353 K using HCl and H 2 SO 4 . A new structured kinetic model was developed that takes into account the difference in the reactivity of β‐(1,4) and β‐(1,3) glycosidic bonds as well as their positions in the polysaccharide chain. To minimize the correlation of adjustable parameters in the new model, the reactivities of these bonds were studied independently (T = 313…363 K; c H+ = 0.1…2 mol/L) using cellobiose and laminaribiose. The difference in kinetic parameters (e.g., T = 338 K: k β‐(1,4) = 0.693 × 10 −3 L/mol/min, k β‐(1,3) = 1.027 × 10 −3 L/mol/min) was found to be statistically significant (P < 0.0001), which emphasizes the need for the structured model for oat β‐glucan hydrolysis. The simulation of β‐glucan hydrolysis with the new model was in good agreement with the experimental data and shows improvement over existing nonstructured models . © 2018 American Institute of Chemical Engineers AIChE J , 64: 2570–2580, 2018