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Enhanced microbial oil production by activated sludge microorganisms via co‐fermentation of glucose and xylose
Author(s) -
Mondala Andro,
Hernandez Rafael,
Holmes William,
French Todd,
McFarland Linda,
Sparks Darrell,
Haque Monica
Publication year - 2013
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.14169
Subject(s) - xylose , fermentation , raw material , chemistry , biodiesel , food science , microorganism , sugar , biofuel , activated sludge , pulp and paper industry , biochemistry , waste management , sewage treatment , microbiology and biotechnology , organic chemistry , bacteria , biology , catalysis , engineering , genetics
The co‐fermentation of glucose and xylose by activated sludge microorganisms for the production of microbial oils for use as biodiesel feedstock was investigated. Various carbon sources at initial concentration of 60 g/L and C:N ratio 70:1 were investigated: xylose, glucose, and 2:1 and 1:2 (by mass) glucose/xylose mixtures. Oil accumulation ranged between 12 to 22% CDW, the highest of which was obtained when xylose was the sole substrate used. Kinetic modeling of the fermentation data showed that specific growth and oil accumulation rates were similar in all substrate types and the lipid coefficient ranged from 0.02 to 0.06 g/g of sugar consumed. The fatty acid methyl ester yield and composition of the lipids showed their suitability for conversion to biodiesel. Based on the results, lignocellulose sugars could be used as fermentation substrates by activated sludge microorganisms for enhancing the oil content of sewage sludge for its use as a sustainable biofuel feedstock source. © 2013 American Institute of Chemical Engineers AIChE J , 59: 4036–4044, 2013