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Electrochemical microreactor for chiral syntheses using the cofactor NADH
Author(s) -
Cheikhou Kane,
Tzédakis Théodore
Publication year - 2008
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.11463
Subject(s) - microreactor , chemistry , electrochemistry , cofactor , redox , nad+ kinase , flavin adenine dinucleotide , biocatalysis , cathode , flavin group , combinatorial chemistry , catalysis , chemical engineering , analytical chemistry (journal) , electrode , organic chemistry , enzyme , engineering
An electrochemical filter‐press microreactor has been designed by electroerosion to perform chiral enzymatic syntheses. Semicylindrical channels are created in the electrodes, only separated by a Nafion membrane. Electrochemical and hydrodynamic characterization of the microreactor allows operating conditions to be optimized. Continuous enzymatic regeneration of NADH was achieved using flavin adenine dinucleotide (FAD/FADH 2 ) as redox mediator. The high specific surface area of the cathode (250 cm −1 ) provides favorable conditions to increase the FADH 2 /FAD concentration ratio and shifts the nonspontaneous reaction FADH 2 /NAD + toward regeneration of NADH. Synthesis of chiral L ‐lactate from pyruvate, in the presence of L ‐LDH, is the model reaction used to check the ability of the microreactor to regenerate NADH; the optimized device allows quantitative yields to be reached in NAD + conversion and in L ‐lactate production. The simulation (FemLab software) results are in good agreement with the experimental mass balances for flow in the range 0.1–0.2 cm 3 /min. © 2008 American Institute of Chemical Engineers AIChE J, 2008