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Skin‐layer formation on porous membrane by immobilized dextransucrase
Author(s) -
Kawakita Hidetaka,
Saito Kyoichi,
Sugita Kazuyuki,
Tamada Masao,
Sugo Takanobu,
Kawamoto Hiroshi
Publication year - 2004
Publication title -
aiche journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 167
eISSN - 1547-5905
pISSN - 0001-1541
DOI - 10.1002/aic.10063
Subject(s) - glycidyl methacrylate , membrane , chemistry , polymer , chemical engineering , polymerization , porosity , monomer , dextransucrase , hollow fiber membrane , polymer chemistry , fiber , permeation , organic chemistry , lactic acid , biochemistry , biology , bacteria , engineering , genetics , leuconostoc mesenteroides
A porous hollow‐fiber membrane with an average pore diameter of 360 nm and a porosity of 71% was used as a starting polymer. An epoxy‐group‐containing monomer, glycidyl methacrylate, was grafted onto the porous hollow‐fiber membrane by radiation‐induced graft polymerization. The produced epoxy group was quantitatively converted into a 2‐hydroxyethylamino group as an anion‐exchange group by reaction with ethanolamine. Dextransucrase solution was forced to permeate radially outward through the pore from the inside surface of the porous hollow‐fiber membrane to be immobilized by the grafted polymer chains based on an anion‐exchange interaction. Subsequently, sucrose solution as substrate was fed to the inside surface of the dextransucrase‐immobilized porous hollow‐fiber membrane. Dextran produced by the enzymatic reaction formed a skin layer on the inside surface of the membrane with a thickness of approximately 4 μm and an estimated pore diameter of 50 nm. © 2004 American Institute of Chemical Engineers AIChE J, 50: 696–700, 2004