Open AccessEvaluation of the expression of bone marrow‐derived mesenchymal stem cells and cancer‐associated fibroblasts in the stroma of gastric cancer tissue
Author(s) -
Minami Taizan,
Aoyagi Keishiro,
Kawahara Akihiko,
Murakami Naotaka,
Isobe Taro,
Tanaka Yuya,
Kaku Hideaki,
Fujita Fumihiko,
Akagi Yoshito
Publication year - 2020
Publication title -
annals of gastroenterological surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.308
H-Index - 15
ISSN - 2475-0328
DOI - 10.1002/ags3.12347
Subject(s) - cancer associated fibroblasts , cancer , stromal cell , pathology , mesenchymal stem cell , cancer cell , bone marrow , medicine , stroma , immunostaining , immunohistochemistry , lymphovascular invasion , metastasis , cancer research
Aim Cancer‐associated fibroblasts (CAFs) generated by bone marrow‐derived mesenchymal stem cells (BM‐MSCs) play an important role in cancer progression. In this study, we investigated the relationships of BM‐MSCs and CAFs in resected gastric cancers with the clinicopathological factors of patients. Methods We analyzed 120 gastric cancer patients who underwent gastrectomy. Immunostaining was performed with an anti‐CD271 antibody (BM‐MSCs) and anti‐α‐smooth muscle actin (αSMA) antibody (CAFs). Staining intensity was used to divide patients into low and high expression groups. Observation sites in cancer tissues were invasive, central, and whole portions. Results Expression of αSMA was significantly related to depth of tumor invasion (T), lymph node metastasis (N), lymphatic invasion (ly), venous invasion (v), and stage. Expression of CD271 was significantly related to v, stage, stromal volume, and tumor infiltration pattern (INF). Overall survival (OS) of the high expression group was significantly lower than that of the low expression group for both αSMA and CD271. Multivariate analysis showed that N, αSMA (whole), and CD271 (invasive) were independent prognostic factors. Conclusions Cancer‐associated fibroblasts and BM‐MSCs are related to the progression, invasion, and prognosis of gastric cancer and may be therapeutic targets of gastric cancer.