Open AccessEffect of bevacizumab plus XELOX (Cape OX ) chemotherapy on liver natural killer cell activity in colorectal cancer with resectable liver metastasis
Author(s) -
Hirata Fumihiro,
Ishiyama Kohei,
Tanaka Yuka,
Kobayashi Tsuyoshi,
Hashimoto Masakazu,
Saeki Yoshihiro,
Ishida Nobuki,
Taguchi Kazuhiro,
Tanaka Junko,
Arihiro Koji,
Ohdan Hideki
Publication year - 2018
Publication title -
annals of gastroenterological surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.308
H-Index - 15
ISSN - 2475-0328
DOI - 10.1002/ags3.12195
Subject(s) - nkg2d , colorectal cancer , peripheral blood mononuclear cell , metastasis , natural killer cell , medicine , chemotherapy , cytotoxic t cell , immune system , lymphokine activated killer cell , bevacizumab , cancer research , immunology , oncology , cancer , interleukin 21 , biology , cd8 , in vitro , biochemistry
Aim We investigated the chemotherapy effect of resectable colorectal cancer with liver metastasis ( CRLM ) on the function of intrahepatic immune cells. Methods We classified patients into adjuvant chemotherapy (bevacizumab+Cape OX ) after hepatectomy group (group A) and neoadjuvant chemotherapy followed by hepatectomy group (group B), and collected peripheral blood mononuclear cells ( PBMC ) and liver mononuclear cells ( LMNC ) to ascertain phenotypic and functional differences. Results There were no significant differences in lymphocyte fractions of either PBMC or LMNC between groups, except for the significantly lower percentage of natural killer ( NK ) cells in LMNC in group B than in group A. Significantly higher percentage of natural‐killer group 2, member D ( NKG 2D)‐ positive NK cells in PBMC and percentage of tumor necrosis factor‐related apoptosis‐inducing ligand ( TRAIL )‐, NK p30‐, and signal regulatory protein β ( SIRP β)‐positive NK cells in LMNC were found in group B. Furthermore, significantly higher expressions of NKG 2D and SIRP β in peripheral blood NK cells and of NK p46 and CD 122 in liver NK cells were found in group B. When LMNC were incubated with interleukin ( IL) ‐2 in vitro, no difference was observed in the expression of these molecules in NK cells between groups. Consistently, there was no difference in the cytotoxic activity of those LMNC against a colon adenocarcinoma cell line between groups. Conclusion Colorectal cancer with liver metastasis patients treated with neoadjuvant chemotherapy showed enhanced expression of activation markers on peripheral blood and liver NK cells in comparison with patients who did not receive therapy; however, the difference in those function remains unclear. These results suggest that neoadjuvant chemotherapy does not have a negative impact on intrahepatic immune cells in resectable CRLM patients.