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Characterization and expression profile analysis of the 3‐phosphoglycerate dehydrogenase family in rice
Author(s) -
Wang Baoxiang,
Guo Tao,
Wang Haifeng,
Li Jingfang,
Sun Zhiguang,
Liu Yan,
Xing Yungao,
Xu Bo,
Yang Bo,
Li Jian,
Chi Ming,
Liu Jinbo,
Chen Tingmu,
Xu Dayong,
Zhu Guanglong
Publication year - 2021
Publication title -
agronomy journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 131
eISSN - 1435-0645
pISSN - 0002-1962
DOI - 10.1002/agj2.20616
Subject(s) - oryza sativa , serine , phosphoglycerate kinase , biology , abiotic component , biochemistry , gene , dehydrogenase , phylogenetic tree , enzyme , paleontology
The plastidial 3‐phosphoglycerate (3‐PGA) pathway plays an important role in supplying serine in non‐photosynthetic tissues in plants under environmental stresses. Synthesis of serine in rice ( Oryza sativa L.) is intangible, but little kinetic and structural information for enzymes is available. In this study, a comprehensive characterization and analysis of phylogenetic variable was carried out on three 3‐phosphoglycerate dehydrogenase (PGDH) members in rice, in addition to an analysis of their whole‐life expression patterns. All of the O ryza sativa phosphoglycerate dehydrogenases ( OsPGDHs ) have three highly conserved motifs. The three genes were detected in all tissues and development stages in ( Oryza sativa , ssp. japonica Nipponbare) by quatitative real‐time polymerase chain reaction (qRT‐PCR). Under abiotic stresses, such as salt, drought, oxidative and low temperature, the expressions of all these OsPGDHs were significantly altered. These results highlighted the importance of the OsPGDH family in phosphorylated serine biosynthesis pathway in rice.