Open AccessGut Microbiome Analysis Can Be Used as a Noninvasive Diagnostic Tool and Plays an Essential Role in the Onset of Membranous Nephropathy
Author(s) -
Shang Jin,
Zhang Yiding,
Guo Ruixue,
Liu Wenli,
Zhang Jun,
Yan Ge,
Wu Feng,
Cui Wen,
Wang Peipei,
Zheng Xuejun,
Wang Ting,
Dong Yijun,
Zhao Jing,
Wang Li,
Xiao Jing,
Zhao Zhanzheng
Publication year - 2022
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202201581
Subject(s) - microbiome , gut microbiome , pathogenesis , biology , gut flora , feces , fecal bacteriotherapy , computational biology , bioinformatics , immunology , microbiology and biotechnology , antibiotics , clostridium difficile
Membranous nephropathy (MN) is a common cause of nephrotic syndrome. The aim is to establish a non‐invasive diagnostic model of MN using differential gut microbiome analysis, and to explore the relationship between the gut microbiome and MN pathogenesis in vivo. 825 fecal samples from MN patients and healthy participants are collected from multiple medical centers across China. Key operational taxonomic units (OTUs) obtained through 16S rRNA sequencing are used to establish a diagnostic model. A rat model of MN is developed to explore the relationship between the gut microbiome and the pathogenesis of MN. The diversity and richness of the gut microbiome are significantly lower in patients with MN than in healthy individuals. The diagnostic model based on seven OTUs achieves an excellent efficiency of 98.36% in the training group and also achieves high efficiency in cross‐regional cohorts. In MN rat model, gut microbiome elimination prevents model establishment, but fecal microbiome transplantation restores the phenotype of protein urine. Gut microbiome analysis can be used as a non‐invasive tool for MN diagnosis. The onset of MN depends on the presence of naturally colonized microbiome. Early intervention in the gut microbiome may help reduce urinary protein level in MN.