Open AccessIntegrated Analytical System for Clinical Single‐Cell Analysis
Author(s) -
Peterson Hannah M.,
Chin Lip Ket,
Iwamoto Yoshi,
Oh Juhyun,
Carlson Jonathan C. T.,
Lee Hakho,
Im Hyungsoon,
Weissleder Ralph
Publication year - 2022
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202200415
Subject(s) - computer science , cancer cell , point of care , biomedical engineering , breast cancer , cancer , computational biology , cancer research , pathology , medicine , biology
High‐dimensional analyses of cancers can potentially be used to better define cancer subtypes, analyze the complex tumor microenvironment, and perform cancer cell pathway analyses for drug trials. Unfortunately, integrated systems that allow such analyses in serial fine needle aspirates within a day or at point‐of‐care currently do not exist. To achieve this, an integrated immunofluorescence single‐cell analyzer (i2SCAN) for deep profiling of directly harvested cells is developed. By combining a novel cellular imaging system, highly cyclable bioorthogonal FAST antibody panels, and integrated computational analysis, it is shown that same‐day analysis is possible in thousands of harvested cells. It is demonstrated that the i2SCAN approach allows comprehensive analysis of breast cancer samples obtained by fine needle aspiration or core tissues. The method is a rapid, robust, and low‐cost solution to high‐dimensional analysis of scant clinical specimens.