Open AccessStabilizing Enzymes in Plasmonic Silk Film for Synergistic Therapy of In Situ SERS Identified Bacteria
Author(s) -
Liu Zhangkun,
Li Shengkai,
Yin Zhiwei,
Zhu Zhaotian,
Chen Long,
Tan Weihong,
Chen Zhuo
Publication year - 2022
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202104576
Subject(s) - photothermal therapy , thermostability , glucose oxidase , bacteria , staphylococcus aureus , antimicrobial , nanotechnology , materials science , chemistry , in situ , silk , antibacterial activity , escherichia coli , microbiology and biotechnology , enzyme , biosensor , biochemistry , biology , organic chemistry , composite material , genetics , gene
Increasing antibiotic resistance becomes a serious threat to public health. Photothermal therapy (PTT) and antibacterial enzyme‐based therapy are promising nonresistant strategies for efficiently killing drug‐resistant bacteria. However, the poor thermostability of enzymes in PTT hinders their synergistic therapy. Herein, antibacterial glucose oxidase (GOx) is embedded in a Ag graphitic nanocapsule (Ag@G) arrayed silk film to fabricate a GOx‐synergistic PTT system (named silk‐GOx‐Ag@G, SGA). The SGA system can stabilize GOx by a vitrification process through the restriction of hydrogen bond and rigid β ‐sheet, and keep the antibacterial activity in the hyperthermal PTT environment. Moreover, the arrayed Ag@G possesses excellent chemical stability due to the protection of graphitic shell, providing stable plasmonic effect for integrating PTT and surface enhanced Raman scattering (SERS) analysis even in the GOx‐produced H 2 O 2 environment. With in situ SERS identification of bacterial intrinsic signals in the mouse wound model, such SGA realizes superior synergistic antibacterial effect on the infected Escherichia coli , Staphylococcus aureus , and methicillin‐resistant Staphylococcus aureus in vivo, while without causing significant biotoxicity. This system provides a therapeutic method with low resistance and in situ diagnosis capability for efficiently eliminating bacteria.