Open AccessEstablishment of Human Pluripotent Stem Cell‐Derived Skin Organoids Enabled Pathophysiological Model of SARS‐CoV‐2 Infection
Author(s) -
Ma Jie,
liu Jia,
Gao Dunqin,
Li Xiao,
Zhang Qiyu,
Lv Luye,
Wang Yujie,
Li Jun,
Zhu Yunping,
Wu Zhihong,
Hu Hengrui,
Li Yufeng,
Ma Longda,
Liu Qian,
Hu Zhihong,
Zhang Shuyang,
Zhou Yiwu,
Wang Manli,
Leng Ling
Publication year - 2022
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202104192
Subject(s) - induced pluripotent stem cell , organoid , stem cell , biology , coronavirus , covid-19 , hair follicle , pathophysiology , human skin , immunology , medicine , pathology , disease , microbiology and biotechnology , infectious disease (medical specialty) , embryonic stem cell , biochemistry , genetics , gene
Coronavirus disease 2019 (COVID‐19) patients with impact on skin and hair loss are reported. Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is detected in the skin of some patients; however, the detailed pathological features of skin tissues from patients infected with SARS‐CoV‐2 at a molecular level are limited. Especially, the ability of SARS‐CoV‐2 to infect skin cells and impact their function is not well understood. A proteome map of COVID‐19 skin is established here and the susceptibility of human‐induced pluripotent stem cell (hiPSC)‐derived skin organoids with hair follicles and nervous system is investigated, to SARS‐CoV‐2 infection. It is shown that KRT17+ hair follicles can be infected by SARS‐CoV‐2 and are associated with the impaired development of hair follicles and epidermis. Different types of nervous system cells are also found to be infected, which can lead to neuron death. Findings from the present work provide evidence for the association between COVID‐19 and hair loss. hiPSC‐derived skin organoids are also presented as an experimental model which can be used to investigate the susceptibility of skin cells to SARS‐CoV‐2 infection and can help identify various pathological mechanisms and drug screening strategies.