Open AccessAptamer Probes Labeled with Lanthanide‐Doped Carbon Nanodots Permit Dual‐Modal Fluorescence and Mass Cytometric Imaging
Author(s) -
Yu Youyi,
Wang Xin,
Jia Xiaolong,
Feng Zijian,
Zhang Lulu,
Li Hongxia,
He Jie,
Shen Guangxia,
Ding Xianting
Publication year - 2021
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202102812
Subject(s) - aptamer , fluorescence , materials science , nanodot , resolution (logic) , chemistry , analytical chemistry (journal) , optics , nanotechnology , physics , microbiology and biotechnology , computer science , chromatography , biology , artificial intelligence
High‐dimensional imaging mass cytometry (IMC) enables simultaneous quantification of over 35 biomarkers on one tissue section. However, its limited resolution and ultralow acquisition speed remain major issues for general clinical application. Meanwhile, conventional immunofluorescence microscopy (IFM) allows sub‐micrometer resolution and rapid identification of the region of interest (ROI), but only operates with low multiplicity. Herein, a series of lanthanide‐doped blue‐, green‐, and red‐fluorescent carbon nanodots (namely, B‐Cdots(Ln 1 ), G‐Cdots(Ln 2 ), and R‐Cdots(Ln 3 )) as fluorescence and mass dual‐modal tags are developed. Coupled with aptamers, B‐Cdots( 159 Tb)‐A10‐3.2, G‐Cdots( 165 Ho)‐AS1411, and R‐Cdots( 169 Tm)‐SYL3C dual‐functional aptamer probes, which are then multiplexed with commercially available Maxpar metal‐tagged antibodies for analyzing clinical formalin‐fixed, paraffin‐embedded (FFPE) prostatic adenocarcinoma (PaC) tissue, are further synthesized. The rapid identification of ROI with IFM using fluorescence signals and subsequent multiplexed detection of in situ ROI with IMC using the same tissue section is demonstrated. Dual‐modal probes save up to 90% IMC blind scanning time for a standard 3.5 mm × 3.5 mm overall image. Meanwhile, the IFM provides refined details and topological spatial distributions for the functional proteins at optical resolution, which compensates for the low resolution of the IMC imaging.