Open AccessFunctionalizing Collagen with Vessel‐Penetrating Two‐Photon Phosphorescence Probes: A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia
Author(s) -
Wu ChengHam,
Kisel Kristina S.,
Thangavel Muthu Kumar,
Chen YiTing,
Chang KaiHsin,
Tsai MingRung,
Chu ChiaYu,
Shen YuFang,
Wu PeiChun,
Zhang Zhiming,
Liu TzuMing,
Jänis Janne,
Grachova Elena V.,
Shakirova Julia R.,
Tunik Sergey P.,
Koshevoy Igor O.,
Chou PiTai
Publication year - 2021
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202102788
Subject(s) - phosphorescence , extravasation , surface modification , in vivo , biomaterial , materials science , two photon excitation microscopy , biophysics , rhenium , reactive oxygen species , chemistry , biomedical engineering , photochemistry , nanotechnology , fluorescence , organic chemistry , pathology , biochemistry , medicine , physics , microbiology and biotechnology , biology , quantum mechanics
The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio‐probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (Re I ) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, Re I ‐diimine complex already exhibits excellent emission yield (34%, λ em = 583 nm) and large two‐photon absorption cross‐sections ( σ 2 = 300 GM @ 800 nm) in water (pH 7.4). After extravasation, remarkably, the collagen‐bound probes further enhanced their excitation efficiency by increasing the deoxygenated lifetime from 4.0 to 7.5 µs, paving a way to visualize tumor hypoxia and tissue ischemia in vivo. The post‐extravasation functionalization of extracellular matrixes demonstrates a new methodology for biomaterial‐empowered phosphorescence sensing and imaging.