Open AccessUltrasound Mediated Cellular Deflection Results in Cellular Depolarization
Author(s) -
Vasan Aditya,
Orosco Jeremy,
Magaram Uri,
Duque Marc,
Weiss Connor,
Tufail Yusuf,
Chalasani Sreekanth H,
Friend James
Publication year - 2022
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202101950
Subject(s) - depolarization , ultrasound , membrane , membrane potential , stimulation , biophysics , electrophysiology , materials science , chemistry , biomedical engineering , neuroscience , biology , medicine , acoustics , biochemistry , physics
Ultrasound has been used to manipulate cells in both humans and animal models. While intramembrane cavitation and lipid clustering have been suggested as likely mechanisms, they lack experimental evidence. Here, high‐speed digital holographic microscopy (kiloHertz order) is used to visualize the cellular membrane dynamics. It is shown that neuronal and fibroblast membranes deflect about 150 nm upon ultrasound stimulation. Next, a biomechanical model that predicts changes in membrane voltage after ultrasound exposure is developed. Finally, the model predictions are validated using whole‐cell patch clamp electrophysiology on primary neurons. Collectively, it is shown that ultrasound stimulation directly defects the neuronal membrane leading to a change in membrane voltage and subsequent depolarization. The model is consistent with existing data and provides a mechanism for both ultrasound‐evoked neurostimulation and sonogenetic control.