Open AccessRejuvenation of Senescent Bone Marrow Mesenchymal Stromal Cells by Pulsed Triboelectric Stimulation
Author(s) -
Li Gaocai,
Zhu Qianqian,
Wang Bingjin,
Luo Rongjin,
Xiao Xiaohui,
Zhang Yi,
Ma Liang,
Feng Xiaobo,
Huang Jingang,
Sun Xuhui,
Wen Zhen,
Pan Yue,
Yang Cao
Publication year - 2021
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202100964
Subject(s) - triboelectric effect , mesenchymal stem cell , stromal cell , stimulation , microbiology and biotechnology , stem cell , senescence , bone marrow , nanogenerator , medicine , neuroscience , cancer research , biology , chemistry , immunology , materials science , piezoelectricity , composite material
Stem cell senescence contributes to stem cell exhaustion and drives various aging‐associated disorders. However, strategies to rejuvenate senescent stem cells are limited. The present study proposes an approach based on triboelectric stimulation to rejuvenate senescent bone marrow mesenchymal stromal cells (BMSCs) by fabricating a pulsed triboelectric nanogenerator (P‐TENG) that can produce stable pulsed current output unaffected by the triggered frequency. The senescence phenotypes of aged BMSCs are reversed by triboelectric stimulation at 30 µA at 1.5 Hz. Triboelectric stimulation enhances the proliferation of aged BMSCs and increases their pluripotency and differentiation capacity. Additionally, mechanistic investigations reveal that pulsed triboelectric stimulation by P‐TENG rejuvenates senescent BMSCs by enhancing MDM2‐dependent p53 degradation, which is demonstrated by loss‐of‐function studies of MDM2 and p53. Overall, this study identifies a new approach for the rejuvenation of senescent BMSCs and describes a promising therapeutic intervention for many diseases associated with aged BMSCs.