Open AccessProduction of a Promising Biosynthetic Self‐Assembled Nanoconjugate Vaccine against Klebsiella Pneumoniae Serotype O2 in a General Escherichia Coli Host
Author(s) -
Peng Zhehui,
Wu Jun,
Wang Kangfeng,
Li Xin,
Sun Peng,
Zhang Lulu,
Huang Jing,
Liu Yan,
Hua Xiaoting,
Yu Yunsong,
Pan Chao,
Wang Hengliang,
Zhu Li
Publication year - 2021
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202100549
Subject(s) - immunogenicity , microbiology and biotechnology , klebsiella pneumoniae , escherichia coli , pathogen , immune system , biology , antigen , virology , immunology , biochemistry , gene
Klebsiella pneumoniae has emerged as a severe opportunistic pathogen with multiple drug resistances. Finding effective vaccines against this pathogen is urgent. Although O‐polysaccharides (OPS) of K. pneumoniae are suitable antigens for the preparation of vaccines given their low levels of diversity, the low immunogenicity (especially serotype O2) limit their application. In this study, a general Escherichia coli host system is developed to produce a nanoscale conjugate vaccine against K. pneumoniae using the Nano‐B5 self‐assembly platform. The experimental data illustrate that this nanoconjugate vaccine can induce an efficient humoral immune response in draining lymph nodes (dLNs) and elicit high titers of the IgG antibody against bacterial lipopolysaccharide (LPS). The ideal prophylactic effects of these nanoconjugate vaccines are further demonstrated in mouse models of both systemic and pulmonary infection. These results demonstrate that OPS with low immunogenicity can be changed into an effective antigen, indicating that other haptens may be applicable to this strategy in the future. To the knowledge, this is the first study to produce biosynthetic nanoconjugate vaccines against K. pneumoniae in E. coli , and this strategy can be applied to the development of other vaccines against pathogenic bacteria.