Open AccessOptogenetic Control of Non‐Apoptotic Cell Death
Author(s) -
He Lian,
Huang Zixian,
Huang Kai,
Chen Rui,
Nguyen Nhung T.,
Wang Rui,
Cai Xiaoli,
Huang Zhiquan,
Siwko Stefan,
Walker Joel R.,
Han Gang,
Zhou Yubin,
Jing Ji
Publication year - 2021
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202100424
Subject(s) - necroptosis , optogenetics , pyroptosis , programmed cell death , microbiology and biotechnology , apoptosis , cell , biology , chemistry , neuroscience , genetics
Herein, a set of optogenetic tools (designated LiPOP) that enable photoswitchable necroptosis and pyroptosis in live cells with varying kinetics, is introduced. The LiPOP tools allow reconstruction of the key molecular steps involved in these two non‐apoptotic cell death pathways by harnessing the power of light. Further, the use of LiPOPs coupled with upconversion nanoparticles or bioluminescence is demonstrated to achieve wireless optogenetic or chemo‐optogenetic killing of cancer cells in multiple mouse tumor models. LiPOPs can trigger necroptotic and pyroptotic cell death in cultured prokaryotic or eukaryotic cells and in living animals, and set the stage for studying the role of non‐apoptotic cell death pathways during microbial infection and anti‐tumor immunity.