Open AccessMicrofluidic Assays: A Precise Microfluidic Assay in Single‐Cell Profile for Screening of Transient Receptor Potential Channel Modulators (Adv. Sci. 11/2020)
Author(s) -
Ai Xiaoni,
Wu Yang,
Lu Wenbo,
Zhang Xinran,
Zhao Lin,
Tu Pengfei,
Wang KeWei,
Jiang Yong
Publication year - 2020
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202070062
Subject(s) - microfluidics , in vivo , cell , transient receptor potential channel , chemistry , transient (computer programming) , receptor , single cell analysis , biophysics , pharmacology , nanotechnology , medicine , materials science , biochemistry , biology , computer science , genetics , operating system
In article number 2000111, Pengfei Tu, KeWei Wang, Yong Jiang, and co‐workers develop a precise microfluidic assay in single‐cell profile for screening of transient receptor potential (TRP) channel modulators. The single‐cell‐based screening strategy dramatically reduces false‐positive/negative results from 76.2% to 4.8% due to full consideration of single‐cell heterogeneity. Four novel coumarin derivatives are cherry‐picked to potently inhibit TRP channels. One of the positive hits, B‐304, reverses TRPA1‐mediated inflammatory pain in vivo.