Open AccessHigh‐Throughput Imaging: Fast, 3D Isotropic Imaging of Whole Mouse Brain Using Multiangle‐Resolved Subvoxel SPIM (Adv. Sci. 3/2020)
Author(s) -
Nie Jun,
Liu Sa,
Yu Tingting,
Li Yusha,
Ping Junyu,
Wan Peng,
Zhao Fang,
Huang Yujie,
Mei Wei,
Zeng Shaoqun,
Zhu Dan,
Fei Peng
Publication year - 2020
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202070015
Subject(s) - clearance , tracing , isotropy , neuroimaging , visualization , computer science , physics , artificial intelligence , optics , neuroscience , biology , medicine , urology , operating system
In article number 1901891, Dan Zhu, Peng Fei, and co‐workers develop an imaging technique, multiangle‐resolved subvoxel selective plane illumination microscopy (termed Mars‐SPIM), that can readily obtain the 3D digital atlas of a cleared whole mouse brain with an isotropic single‐cell resolution, in a short acquisition time of several minutes. It provides an efficient way to implement system‐level cellular analysis, such as the mapping of different neuron populations and tracing of long‐distance neural projections over the entire brain.